IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/07/20: Difference between revisions
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pSC001 is also a positive control, as I know that the plasmid works. | pSC001 is also a positive control, as I know that the plasmid works. | ||
I made a culture in 7,5mL of LB + 7,5µL Amp at 10:00 a.m., in order to miniprep around | I made a culture in 7,5mL of LB + 7,5µL Amp at 10:00 a.m., in order to miniprep around 4 pm., or before if possible. | ||
==Miniprep of pSC001 & pSC003== | |||
* Usual protocol, I recover DNA in 50 µL of water. | |||
* Nanodrop : | |||
** pSC001 : 54 ng/µL | |||
** pSC003 : 151 ng/µL | |||
== Digestion of pSC003 == | |||
* DNA : 20 µL | |||
* XbaI : 2 µL | |||
* PstI : 2µL | |||
* FD buffer 10X : 4 µL | |||
* water : 12µL | |||
* Gel purification | |||
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Revision as of 10:38, 20 July 2012
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Result transformation pSC001 (R0011, pLac) & pSC003 (K228001, supD)
pSC001 is also a positive control, as I know that the plasmid works. I made a culture in 7,5mL of LB + 7,5µL Amp at 10:00 a.m., in order to miniprep around 4 pm., or before if possible. Miniprep of pSC001 & pSC003
Digestion of pSC003
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