IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/07/24: Difference between revisions
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Here is the content of 1 tube of PCR : | Here is the content of 1 tube of PCR : | ||
VR (diluted 10 times from the stock tube) : 2,5µL | *VR (diluted 10 times from the stock tube) : 2,5µL | ||
VF2 (diluted 10 times from the stock tube) : 2,5µL | *VF2 (diluted 10 times from the stock tube) : 2,5µL | ||
PCR MM 2X : 25 µL | *PCR MM 2X : 25 µL | ||
water : 20 µL | *water : 20 µL | ||
Here is the PCR pgm : | Here is the PCR pgm : | ||
95°C - 15' (to lyse cells too) | *95°C - 15' (to lyse cells too) | ||
95°C - 30" | *95°C - 30" | ||
50°C - 30" | *50°C - 30" | ||
72°C - 40" | *72°C - 40" | ||
72°C - 10' | *72°C - 10' | ||
4°C -<math>\infty</math> | *4°C -<math>\infty</math> | ||
-> 30 cycles | -> 30 cycles | ||
Revision as of 04:49, 27 July 2012
 Project name
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Result Transformation
I took 5 clones from L11 1/20 plate, and 2 clones from the vector only plate, and I make PCR and a culture of these colonies : Here is the content of 1 tube of PCR :
Here is the PCR pgm :
-> 30 cycles The culture tube are composed of 10mL of LB + 10µL Amp -80°C stockI made a -80°C stock for the transformation from yesterday (#12->#22) Results SequencingAll plasmids sequenced so far are ok. |
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