IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/08/21: Difference between revisions

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<!--* I transform them in NEB turbo, competent cells, along with the miniprep of BB R0011 (pSC001)
* I transform them in NEB turbo, competent cells
I transform 0.5 µL of plasmid in 20 µL of cells.  
I transform 1 µL of plasmid in 20 µL of cells.  
** Let 30' on ice  
** Let 30' on ice  
** heat shock 42°C
** heat shock 42°C
** 2' on ice
** 20' on ice
** add 200 µL of warm LB (pre-heated at 42°C)
** add 180 µL of LB
** shaking at 37°C for 1h
** shaking at 37°C for 1h
** Spread 20 µL and 180 µL in LB+Amp or L+Amp+Kan (K228001) plates
** Spread 20 µL and 180 µL in LB+Amp or L+Amp+Kan (K228001) plates
** O/N 37°C
** O/N 37°C


As positive control, I transform in the same condition the R0011 which has already been shown to be a good plasmid (pSB1A2)
 
As negative control, I just spread the NEB turbo without adding any DNA.
-->
-->
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__NOTOC__
[[category:OWWLabNotebookV1]]
[[category:OWWLabNotebookV1]]

Revision as of 11:42, 21 August 2012

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Biobricks retrieval : P1003

Parts number Plasmid Name Distribution/Plate/Well
BBa_K228001 KanR pSB1A1 2012/1/5P
  • I transform them in NEB turbo, competent cells

I transform 1 µL of plasmid in 20 µL of cells.

    • Let 30' on ice
    • heat shock 42°C
    • 20' on ice
    • add 180 µL of LB
    • shaking at 37°C for 1h
    • Spread 20 µL and 180 µL in LB+Amp or L+Amp+Kan (K228001) plates
    • O/N 37°C


-->

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