IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/08/23: Difference between revisions
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I have few colonies on the plate on which I plate all, and no colonies on which I plate 200µL at 1/20 dilution. I have a lot with the positive control, and none with negative control . Anyway, I made 2 clones in culture at 11:00 am. The culture is 10mL of LB + 10µL of Cm. I'll miniprep this culture around 5 pm. | I have few colonies on the plate on which I plate all, and no colonies on which I plate 200µL at 1/20 dilution. I have a lot with the positive control, and none with negative control . Anyway, I made 2 clones in culture at 11:00 am. The culture is 10mL of LB + 10µL of Cm. I'll miniprep this culture around 5 pm. | ||
==Miniprep of pSC009-1&2== | |||
* Usual protocol, I recover DNA in 50 µL of water. | |||
* Nanodrop : | |||
** pSC009-1 : 170 ng/µL | |||
** pSC009-2 : 131 ng/µL | |||
Revision as of 09:56, 23 August 2012
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Result transformationI have few colonies on the plate on which I plate all, and no colonies on which I plate 200µL at 1/20 dilution. I have a lot with the positive control, and none with negative control . Anyway, I made 2 clones in culture at 11:00 am. The culture is 10mL of LB + 10µL of Cm. I'll miniprep this culture around 5 pm. Miniprep of pSC009-1&2
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