IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/08/25: Difference between revisions
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==Digestion of | ==Digestion of pSC008== | ||
* | * pSC008-1 (301ng/µL) : EcoRI + PstI | ||
** DNA : 45 µL / 3 / 3 | ** DNA : 45 µL / 3 / 3 | ||
** EcoRI : 14 µL / 1 / 0 | ** EcoRI : 14 µL / 1 / 0 | ||
Revision as of 02:24, 27 August 2012
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Miniprep of the O/N culture of pSC008I use the thermoscientific kit, but with the vacuum method. After Centrifugation, resuspension, lysis, neutralization, centrifugation for 5', I plug the column on the vacuum machin, and then add the supernatant, switch on the vacuum, let the liquid pass through. Once there is no more liquid, I add 700μL of wash solution, twice. I centrifuge 1' to remove ethanol, and I finally elute in 50μL of water.
Digestion of pSC008
--> 120 min, 37°C water bath. Migration of the Digestion | |
