IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/08/30: Difference between revisions
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It didn't work well. This morning I had no colonies on no plates. But it turns out that few colonies appeared in the afternoon. | It didn't work well. This morning I had no colonies on no plates. But it turns out that few colonies appeared in the afternoon. | ||
I make them grow in 10ml of LB+Amp and in LB+Kan | |||
On the pUC control, there are about 20 colonies | On the pUC control, there are about 20 colonies |
Revision as of 03:02, 7 September 2012
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Result QCMIt didn't work well. This morning I had no colonies on no plates. But it turns out that few colonies appeared in the afternoon. I make them grow in 10ml of LB+Amp and in LB+Kan On the pUC control, there are about 20 colonies I run a gel with 10µL of the digested DNA to check wether the PCR and digestion worked well.
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