IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/09/03: Difference between revisions

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I make culture of all colonies expected (14 overall, 1(Cm) + 6+7 (Amp)), then I put the plate back to the 37°C incubator.


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Revision as of 05:25, 3 September 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Result transformation

Which plate Number of Colonies Plates
L1 1 200µL pur Cm
L2 0 200µL pur Cm
L3 0 200µL pur
positive transformation control Cm (pSB1C3) .+++ 200µL Cm
negative Cm transformation control (NEB only) 0 200µL Cm
negative ligation control (pSC010 dig. ie. vector) 0 200µL Cm
Positive ligation control (pSC001dig+L11dig) .+++ 200µL (Amp)
negative Amp transformation control (NEB only) 0 200µL (Amp)
positive transformation control Amp (pSC001) .+++ 200µL Amp
#####QCM transformation##### ##### #####
QCM-1 6 200µL Amp
QCM-2 7 200µL Amp
QCM-3 0 200µL Amp
QCM-Control 1 200µL Amp IPTG Xgal
pUC18 .++ 200µL Amp IPTG Xgal

I make culture of all colonies expected (14 overall, 1(Cm) + 6+7 (Amp)), then I put the plate back to the 37°C incubator.