IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/09/07: Difference between revisions
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(Autocreate 2012/09/07 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/Semantic_group/day_by_day/) |
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== | ==Result of transformation== | ||
Positive and negative control are as expected. And I have colonies as expected on plate. I make them grow in liquid culture. I picked 2 of each, and put them in LB+Cm, in order to make competent cell with them, but also -80°C stock. | |||
{| border = "1" width="45%" | |||
! width="15%" | -80°C stock number | |||
! width="25%" | strain | |||
! width="15%" | Backbone | |||
! width="15%" | old name | |||
! width="15%" | New name | |||
|- | |||
|61 | |||
|NEB turbo | |||
|pSB1C3 | |||
|L11 | |||
|pSC011 | |||
|- | |||
|62 | |||
|XL1-blue | |||
|pSB1A1 | |||
|QCM-1a | |||
|pSC015 | |||
|- | |||
|63 | |||
|XL1-blue | |||
|pSB1A1 | |||
|QCM-3b | |||
|pSC015 | |||
|- | |||
|64 | |||
|MG1655 | |||
|pSB1A1 | |||
|QCM-1a | |||
|pSC012 | |||
|- | |||
|65 | |||
|MG1655 | |||
|pSB1A1 | |||
|QCM-3b | |||
|pSC012 | |||
|- | |||
|## | |||
|MG1655 | |||
|pSB1C3 | |||
|pSB1C3::RFP | |||
|pSC013 | |||
|- | |||
|## | |||
|MG1655 | |||
|pSB1C3 | |||
|pSB1C3::supD | |||
|pSC014 | |||
|- | |||
|} | |||
Revision as of 03:37, 7 September 2012
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Result of transformationPositive and negative control are as expected. And I have colonies as expected on plate. I make them grow in liquid culture. I picked 2 of each, and put them in LB+Cm, in order to make competent cell with them, but also -80°C stock.
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