IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/09/07: Difference between revisions

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Positive and negative control are as expected. And I have colonies as expected on plate. I make them grow in liquid culture. I picked 2 of each, and put them in LB+Cm, in order to make competent cell with them, but also -80°C stock.
Positive and negative control are as expected. And I have colonies as expected on plate. I make them grow in liquid culture. I picked 2 of each, and put them in LB+Cm, in order to make competent cell with them, but also -80°C stock.
==-80°c stock and new names==


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Revision as of 03:38, 7 September 2012

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Result of transformation

Positive and negative control are as expected. And I have colonies as expected on plate. I make them grow in liquid culture. I picked 2 of each, and put them in LB+Cm, in order to make competent cell with them, but also -80°C stock.


-80°c stock and new names

-80°C stock number strain Backbone old name New name
61 NEB turbo pSB1C3 L11 pSC011
62 XL1-blue pSB1A1 QCM-1a pSC015
63 XL1-blue pSB1A1 QCM-3b pSC015
64 MG1655 pSB1A1 QCM-1a pSC012
65 MG1655 pSB1A1 QCM-3b pSC012
## MG1655 pSB1C3 pSB1C3::RFP pSC013
## MG1655 pSB1C3 pSB1C3::supD pSC014