IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/09/08: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 10: | Line 10: | ||
>Oligo_AmpR_2_Fw_QCM Tm=85.59 mut=1 | >Oligo_AmpR_2_Fw_QCM Tm=85.59 mut=1 | ||
GGACCACTTCTGCGCTAGGCCCTTCCGGCTGG | GGACCACTTCTGCGCTAGGCCCTTCCGGCTGG | ||
>Oligo_AmpR_2_Rev_QCM Tm=85.59 | >Oligo_AmpR_2_Rev_QCM Tm=85.59 | ||
CCAGCCGGAAGGGCCTAGCGCAGAAGTGGTCC | CCAGCCGGAAGGGCCTAGCGCAGAAGTGGTCC | ||
>Oligo_AmpR_Fw_PCR_QCM Tm=79.18 mut=2 | >Oligo_AmpR_Fw_PCR_QCM Tm=79.18 mut=2 | ||
GGCGCGGTATTATAGCGTATTGACGCCGGGC | GGCGCGGTATTATAGCGTATTGACGCCGGGC | ||
>Oligo_AmpR_Rev_PCR_QCM Tm=79.18 | >Oligo_AmpR_Rev_PCR_QCM Tm=79.18 | ||
GCCCGGCGTCAATACGCTATAATACCGCGCC | GCCCGGCGTCAATACGCTATAATACCGCGCC | ||
>Oligo_CmR_Fw_QCM Tm=79.44 mut=3 | >Oligo_CmR_Fw_QCM Tm=79.44 mut=3 | ||
CCATGAGCAAACTGAAACGTTTTAGTAGCTCTGGAGTGAATACCACG | CCATGAGCAAACTGAAACGTTTTAGTAGCTCTGGAGTGAATACCACG | ||
>Oligo_CmR_Rev_QCM Tm=79.44 | >Oligo_CmR_Rev_QCM Tm=79.44 | ||
CGTGGTATTCACTCCAGAGCTACTAAAACGTTTCAGTTTGCTCATGG | CGTGGTATTCACTCCAGAGCTACTAAAACGTTTCAGTTTGCTCATGG | ||
>Oligo_KanR_2_Fw_QCM Tm=82.025 mut=2 | >Oligo_KanR_2_Fw_QCM Tm=82.025 mut=2 | ||
CGTCTTGCTCAGGCGCAATAGCGCATGAATAACGGTTTGG | CGTCTTGCTCAGGCGCAATAGCGCATGAATAACGGTTTGG | ||
>Oligo_KanR_2_Rev_QCM Tm=82.025 | >Oligo_KanR_2_Rev_QCM Tm=82.025 | ||
CCAAACCGTTATTCATGCGCTATTGCGCCTGAGCAAGACG | CCAAACCGTTATTCATGCGCTATTGCGCCTGAGCAAGACG | ||
>Oligo_TetR_Fw_QCM Tm=79.53 mut=5 | >Oligo_TetR_Fw_QCM Tm=79.53 mut=5 | ||
GCGGGATATCGTCCATTAGGACTAGATCGCCAGTCACTATGGCGTGC | GCGGGATATCGTCCATTAGGACTAGATCGCCAGTCACTATGGCGTGC | ||
>Oligo_TetR_Rev_QCM Tm=79.53 | >Oligo_TetR_Rev_QCM Tm=79.53 | ||
GCACGCCATAGTGACTGGCGATCTAGTCCTAATGGACGATATCCCGC | GCACGCCATAGTGACTGGCGATCTAGTCCTAATGGACGATATCCCGC | ||
I use the following code (python) to determine Tm. the equation is given in the kit. | I use the following code (python) to determine Tm. the equation is given in the kit. |
Revision as of 03:38, 8 September 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
New primers QCMI ordered the following primers in order to mutate 2 serines in the AmpR,TetR,CmR and KanR gene. >Oligo_AmpR_2_Fw_QCM Tm=85.59 mut=1 GGACCACTTCTGCGCTAGGCCCTTCCGGCTGG >Oligo_AmpR_2_Rev_QCM Tm=85.59 CCAGCCGGAAGGGCCTAGCGCAGAAGTGGTCC >Oligo_AmpR_Fw_PCR_QCM Tm=79.18 mut=2 GGCGCGGTATTATAGCGTATTGACGCCGGGC >Oligo_AmpR_Rev_PCR_QCM Tm=79.18 GCCCGGCGTCAATACGCTATAATACCGCGCC >Oligo_CmR_Fw_QCM Tm=79.44 mut=3 CCATGAGCAAACTGAAACGTTTTAGTAGCTCTGGAGTGAATACCACG >Oligo_CmR_Rev_QCM Tm=79.44 CGTGGTATTCACTCCAGAGCTACTAAAACGTTTCAGTTTGCTCATGG >Oligo_KanR_2_Fw_QCM Tm=82.025 mut=2 CGTCTTGCTCAGGCGCAATAGCGCATGAATAACGGTTTGG >Oligo_KanR_2_Rev_QCM Tm=82.025 CCAAACCGTTATTCATGCGCTATTGCGCCTGAGCAAGACG >Oligo_TetR_Fw_QCM Tm=79.53 mut=5 GCGGGATATCGTCCATTAGGACTAGATCGCCAGTCACTATGGCGTGC >Oligo_TetR_Rev_QCM Tm=79.53 GCACGCCATAGTGACTGGCGATCTAGTCCTAATGGACGATATCCCGC I use the following code (python) to determine Tm. the equation is given in the kit. def Tm(N=N,gc=gc,mut=mut): Tm = 81.5 + 0.41*gc-675/N-((mut*100.0)/N) return Tm Where N is the number of bp, gc the % of GC, and mut, the number of mutation added. |