IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/09/17: Difference between revisions
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|200 | |200 | ||
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|200µL pur | |200µL pur & 1/100 Cm + Kan | ||
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|3b (plasmid from #63, P1003 TCG->TAG) | |3b (plasmid from #63, P1003 TCG->TAG) | ||
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|200 | |200 | ||
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|200µL 1/100 Cm + Kan | |200µL pur & 1/100 Cm + Kan | ||
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Revision as of 02:34, 18 September 2012
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Electro competent cellAfter the ON culture, I diluted the pSC013 & pSC014 culture 100 times in LB + Cm. I wait until the OD600 reach 0.740, then I follow the protocol for eletrocompetent cell. [Link] I add another washing step because, I vortexed once which could have release salt. Transformation
The pulse time for :
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