IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/09/18: Difference between revisions
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As expected, the supD construction can rescue the lost of resistance to Kanamycin. This part is qualitatively good. | As expected, the supD construction can rescue the lost of resistance to Kanamycin. This part is qualitatively good. | ||
-> Need to create new page in the part registry. | -> Need to create new page in the part registry. : Done : http://partsregistry.org/Part:BBa_K914000 | ||
==QCM on backbones== | |||
I did the QCM without the kit, with a Pfu Ultra from Fermentas and DpnI from fermentas. Nevertheless, I followed the protocol, and time recommendation. | |||
For each Backbone, I have 2 samples and 1 control, which is without template (i.e. backbone) | |||
I add 5 µL of buffer 10X of FD DpnI. | |||
Then I transform in NEB turbo cell that carry an amber suppressor. I transformed 4 times from each sample 5µL in 20µL of cell. | |||
The positive control is the plasmid I used before the PCR. | |||
So for each antibiotic I have 8 samples and 2 controls. | |||
Revision as of 16:31, 18 September 2012
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Result Transformation
As expected, the supD construction can rescue the lost of resistance to Kanamycin. This part is qualitatively good. -> Need to create new page in the part registry. : Done : http://partsregistry.org/Part:BBa_K914000 QCM on backbonesI did the QCM without the kit, with a Pfu Ultra from Fermentas and DpnI from fermentas. Nevertheless, I followed the protocol, and time recommendation. For each Backbone, I have 2 samples and 1 control, which is without template (i.e. backbone) I add 5 µL of buffer 10X of FD DpnI. Then I transform in NEB turbo cell that carry an amber suppressor. I transformed 4 times from each sample 5µL in 20µL of cell. The positive control is the plasmid I used before the PCR. So for each antibiotic I have 8 samples and 2 controls. |