IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/10/16
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PCR QCM - 2nd part
I plated everything on LB+Amp, and let it grow O/N at 37°C. Soil sample analysisI took 3 sample of soil from the sidewalk, at 3 different places, that weight about 20-30g. I add 25mL of distilled water and vortex hard for couples of minutes. I let settled over night. This morning I took in duplicate 50µL, 25µL, 15µL, 5µL of supernatant for sample. I add water up to 50µL. I put this at 99°C for 10min, then I spin it down for 5min, and I took 5µL of the supernatant. So, I ran the PCR with : • 5µL of supernatant • 15µL of water • 2,5µL of primers (VF2 & VR) • 25µL of PCR MM (contain taq, dNTP, ..) PCR pgm :
-> 30 cycles
Soil sample test |