IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/10/22
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Cloning Kan** into pSB1C3Fail after gel purification. Preparation of the Tecan experiment with Kan**Since the sequence of QCM are good (we have a Kan gene with 2 amber mutation), we can prepare the Tecan experiment to quantify the leakiness of the system. Double transformationWe to double transform this new Kan** gene with either supD or RFP : I transformed in 20µL of MG1655 competent cells, 1.5µL of the miniprep of Kan** (From S1-1) and 1.5µL of pSC011 (supD) in triplicate. I did the same but with pSB1C3::RFP instead of supD. I also transformed this 3 plasmids alone, to test them. I have a negative control, which is only cell, without plasmid.
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