IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/10/16

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(PCR QCM - 2nd part)
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==PCR QCM - 2nd part==
==PCR QCM - 2nd part==
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* I digested the PCR product with DpnI for 1h, then I transformed either 1, 3 or 5µL of digested product in NEB and MG1655 cells.
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* I add 200µL of LB and then let recovering at 37°C for 1h.
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I plated everything on LB+Amp, and let it grow O/N at 37°C.
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==Soil sample test==

Revision as of 11:37, 16 October 2012

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PCR QCM - 2nd part

  • I digested the PCR product with DpnI for 1h, then I transformed either 1, 3 or 5µL of digested product in NEB and MG1655 cells.
  • I add 200µL of LB and then let recovering at 37°C for 1h.

I plated everything on LB+Amp, and let it grow O/N at 37°C.

Soil sample test

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