IGEM:Paris Bettencourt 2012/Notebooks/Semantic group/day by day//2012/10/23

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(Autocreate 2012/10/23 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/Semantic_group/day_by_day/)
Current revision (05:38, 24 October 2012) (view source)
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==Result Transformation==
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I have one clone for supD+Kan** and 4 clones for RFP+Kan** but only one is red under UV.
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I make them grow in 3 differents tubes of 10mL of LB+10µL of Cm+10µL of Amp.
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I also made culture of supD+KanR (same condition with 10µL of Kan) from the 3 -80°C tubes, same for pLac+supD in Cm+Amp from the -80°C stock.
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Result Transformation

I have one clone for supD+Kan** and 4 clones for RFP+Kan** but only one is red under UV.

I make them grow in 3 differents tubes of 10mL of LB+10µL of Cm+10µL of Amp.

I also made culture of supD+KanR (same condition with 10µL of Kan) from the 3 -80°C tubes, same for pLac+supD in Cm+Amp from the -80°C stock.


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