IGEM:Paris Bettencourt 2012/Notebooks/modularity group: Difference between revisions
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===the circuit=== | ===the circuit=== | ||
[[Image:Bristol-construct.png|border|left|500px|]] more details in the future | [[Image:Bristol-construct.png|border|left|500px|]] more details in the future | ||
=Materials= | |||
==E coli strains== | |||
'''DH5α Turbo (NEB)''': the cells that we have. | |||
F´ proA+B+ lacIq ∆ lacZ M15/ fhuA2 ∆(lac-proAB) glnV gal R(zgb-210::Tn10)TetS endA1 thi-1 ∆(hsdS-mcrB)5 | |||
Also known as NEB Turbo | |||
T1 phage resistant | |||
Rapid growth: visible colonies on agar, ~6.5 hours; shaking liquid culture OD 600 = 2.0, ~4 hours | |||
Expresses the Lac repressor | |||
References: | |||
New England Biolabs, product catalogue number C2984H | |||
Revision as of 06:34, 3 July 2012
Purpuse of this group
Projects
PKU 2010 [1]
concept
the circuit
comment on the modularity
Cambridge 2010 [2]
concept
the circuit
comment on the modularity
Bristol 2010 [3]
encapsulation technique:[ http://www.freepatentsonline.com/5093253.html]
concept
the circuit
more details in the future
Materials
E coli strains
DH5α Turbo (NEB): the cells that we have.
F´ proA+B+ lacIq ∆ lacZ M15/ fhuA2 ∆(lac-proAB) glnV gal R(zgb-210::Tn10)TetS endA1 thi-1 ∆(hsdS-mcrB)5 Also known as NEB Turbo
T1 phage resistant Rapid growth: visible colonies on agar, ~6.5 hours; shaking liquid culture OD 600 = 2.0, ~4 hours Expresses the Lac repressor References: New England Biolabs, product catalogue number C2984H
comment on the modularity
What we are doing now
PKU 2010
communication, requests of plasmids. searching for mercury, it seems that we need 2 strains: BL21DE3 and DH5 alpha depending of the part of the project we want to do. (both?)
Cambridge 2010
We have the biobrick we want to use (but there are 2 in fact): 2012 Kit Plate 4 well 17M, resistance: C But should we go on with that project? it uses the pBad promoter.
Bristol 2010
We have the biobrick: complete system: 2012 Kit Plate 4 well 1A, resistance: C
finding nitrates?
