IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/05: Difference between revisions
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(Autocreate 2012/07/05 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/modularity_group/day_by_day/) |
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== | ==Transformation Efficiency of NEB Turbo (28/06/2012) Chemically Competent Cells== | ||
* | * 10^5 transformants/ug | ||
* Started 15 mL culture in the morning of NEB Turbo with pSB1C3 (pMG.003) | |||
==Result of K325909 transformation of 4/7/12== | |||
* 1 colony on the plate with 200 uL transformed culture added | |||
* Started 15 mL culture in the morning of NEB Turbo with pSB1C3 (pMG.002) | |||
==Glycerol stocks and Minipreps of 3 X K381001 (pMG.001) Clones== | |||
* Stocks made in duplicate (-20C and -80C) of 5 mL liquid cultures of the 3 clones of pMG.001 from 04/07/2012 | |||
* Minipreps of remaining culture | |||
{| class="wikitable" | |||
{| border="1" | |||
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! Culture # !! Conc. DNA | |||
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| C1 || 119 ng/uL | |||
|- | |||
| C2 || 141 ng/uL | |||
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| C3 || 122 ng/uL | |||
|} | |||
Revision as of 06:11, 5 July 2012
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Transformation Efficiency of NEB Turbo (28/06/2012) Chemically Competent Cells
Result of K325909 transformation of 4/7/12
Glycerol stocks and Minipreps of 3 X K381001 (pMG.001) Clones
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