IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/17: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Biobrick BBa_I716462==
in accordance with discussion with Yifan,
in accordance with discussion with Yifan,
we want to use the BamH1Bekerley 2007/ Letherbridge 2011 project.[[ LINK]]
we want to use the BamH1Bekerley 2007/ Letherbridge 2011 project.[[ LINK]]
Line 29: Line 29:
( it is registered in the "online following sheet")
( it is registered in the "online following sheet")


==Transformation==


* of pMG.004, AKA BBa_I716462
* Following jake's protocol
{| class="wikitable"
{| border="1"
|-
! Plate !! Plasmid !! Dilution
|-
| 1 || BBa_I716462 from registry || 1:1
|-
| 2 || BBa_I716462 from registry || 1:10
|-
| 3 || lacZ on pSB1A2 108 ng/uL  || 1:1
|-
| 4 || lacZ on pSB1A2 108 ng/uL  || 1:10
|}


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 10:02, 17 July 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Biobrick BBa_I716462

in accordance with discussion with Yifan, we want to use the BamH1Bekerley 2007/ Letherbridge 2011 project.LINK

opening of a new biobrick:

Code Date Received Registry Markers Rep. Origin Backbone Source Description of Function Location Notes
pMG.004 17-07-12 BBa_I716462 A pMB1 pSB1A2 2012 Kit Plate 2 3C BamHI endonuclease under pBAD

( it is registered in the "online following sheet")

Transformation

  • of pMG.004, AKA BBa_I716462
  • Following jake's protocol
Plate Plasmid Dilution
1 BBa_I716462 from registry 1:1
2 BBa_I716462 from registry 1:10
3 lacZ on pSB1A2 108 ng/uL 1:1
4 lacZ on pSB1A2 108 ng/uL 1:10
Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Paris_Bettencourt_2012/Notebooks/modularity_group/day_by_day//2012/07/17&oldid=614500"