IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/18: Difference between revisions
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Presence of .... colonies in the 1/1 petri dish. Nothing in the 1/10. | Presence of .... colonies in the 1/1 petri dish. Nothing in the 1/10. | ||
== | ==miniprepping and glycerol stocks of pMG.004== | ||
'''12h: launching of 12mL cultures in 50 mL flasks''': | '''12h: launching of 12mL cultures in 50 mL flasks''': | ||
3 cultures from 3 distinct colonies of pmg.004/NEB turbo culture. (LB + 12μL Ampicilline) | 3 cultures from 3 distinct colonies of pmg.004/NEB turbo culture. (LB + 12μL Ampicilline) | ||
Miniprep plasmid stock | |||
{| class="wikitable" | |||
{| border="1" | |||
|- | |||
! Clone !! Concentration [ng/uL] | |||
|- | |||
| C1 || 92.7 | |||
|- | |||
| C2 || 121.8 | |||
|- | |||
| C3 || 126.8 | |||
|} | |||
==Glycerol stocks of TOP10== | |||
3 cultures of top 10.( LB + 6μL Spectomycine) | 3 cultures of top 10.( LB + 6μL Spectomycine) | ||
3 glycerol stocks of each (1 each for Chantal, 2 for us) | |||
Slow growing culture, too much antibiotic? | |||
==Launched culture for TOP10 competent cells== | |||
*20mL with Streptomycin | |||
==discution with Julien: 2nd round== | |||
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__NOTOC__ | __NOTOC__ | ||
[[category:OWWLabNotebookV1]] | [[category:OWWLabNotebookV1]] | ||
Revision as of 06:37, 19 July 2012
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transformation resultscontrol OK. Presence of .... colonies in the 1/1 petri dish. Nothing in the 1/10. miniprepping and glycerol stocks of pMG.00412h: launching of 12mL cultures in 50 mL flasks: 3 cultures from 3 distinct colonies of pmg.004/NEB turbo culture. (LB + 12μL Ampicilline) Miniprep plasmid stock
Glycerol stocks of TOP103 cultures of top 10.( LB + 6μL Spectomycine) 3 glycerol stocks of each (1 each for Chantal, 2 for us) Slow growing culture, too much antibiotic? Launched culture for TOP10 competent cells
discution with Julien: 2nd round | |||||||||
