IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/18

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transformation results

control OK. Presence of .... colonies in the 1/1 petri dish. Nothing in the 1/10.

miniprepping and glycerol stocks of pMG.004

12h: launching of 12mL cultures in 50 mL flasks: 3 cultures from 3 distinct colonies of pmg.004/NEB turbo culture. (LB + 12μL Ampicilline)

Miniprep plasmid stock

Clone Concentration [ng/uL]
C1 92.7
C2 121.8
C3 126.8

Glycerol stocks of TOP10

3 cultures of top 10.( LB + 6μL Spectomycine) 3 glycerol stocks of each (1 each for Chantal, 2 for us) Slow growing culture, too much antibiotic?

Launched culture for TOP10 competent cells

  • 20mL with Streptomycin

discution with Julien: 2nd round

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