IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/19: Difference between revisions
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Latest revision as of 10:05, 20 July 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> back to our group notebook <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||
preparing gene transfer assayFollowing Juliens advices and references (....), we founded that b. Subtilis was a good candidate for our gene transfer assay. Even more since we had already strains: there are 3 strains in IGEM2011 glycerol stocks:
we will use S10 or S11. But Baback is worried that BSPY79 is contaminated. To ckeck it and to obtain a non contaminated new glycerol stock we searched for singles on ... plates: - - - - - - Analytical digest of pMG.004 miniprep from 18/7/12Expected:
5uL digest with XbaI and PstI 3xMinipreps C1, C2, C3
Re-doing analytical digest of pMG.004
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