IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/19: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 14: | Line 14: | ||
Baback is worried that BSPY79 is contaminated. | Baback is worried that BSPY79 is contaminated. | ||
To ckeck it and to obtain a non contaminated new glycerol stock; | |||
==Analytical digest of pMG.004 miniprep from 18/7/12== | ==Analytical digest of pMG.004 miniprep from 18/7/12== | ||
Expected: | |||
* 2020 bp backbone pST1A2 | |||
* 1940 bp insert BBa_I716462 | |||
5uL digest with XbaI and PstI 3xMinipreps C1, C2, C3 | |||
[[image:KR003829.png]] | |||
*Bad result, what is the problem? It's as though it is 1/2 digested but the size is too small (would be 4kb) | |||
==Re-doing analytical digest of pMG.004== | |||
* Only of C1 miniprep from 18/7/12 | |||
* | |||
{| class="wikitable" | |||
{| border="1" | |||
|- | |||
! !! XbaI !! PstI | |||
|- | |||
| 1 || X || | |||
|- | |||
| 2 || || X | |||
|- | |||
| 3 || X || X | |||
|- | |||
| 4 || || | |||
|} | |||
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### --> |
Revision as of 09:41, 19 July 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> back to our group notebook <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||
preparing gene transfer assayFollowing Juliens advices and references (....), we founded that b. Subtilis was a good candidate for our gene transfer assay. Even more since we had already strains: there are 3 strains in IGEM2011 glycerol stocks: BSPY79: wild type. SB513: Kan resistant. (stored as S10) SB473: CM resistant. (stroed as S11) Baback is worried that BSPY79 is contaminated. To ckeck it and to obtain a non contaminated new glycerol stock; Analytical digest of pMG.004 miniprep from 18/7/12Expected:
5uL digest with XbaI and PstI 3xMinipreps C1, C2, C3
Re-doing analytical digest of pMG.004
| ||||||||||||||||