IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/19: Difference between revisions
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5uL digest with XbaI and PstI 3xMinipreps C1, C2, C3 | 5uL digest with XbaI and PstI 3xMinipreps C1, C2, C3 | ||
[[image:KR003829.png]] | [[image:KR003829.png]] | ||
*Bad result, what is the problem? It's as though it is 1/2 digested but the size is too small (would be 4kb) | *Bad result, what is the problem? It's as though it is 1/2 digested but the size is too small (would be 4kb) | ||
==Re-doing analytical digest of pMG.004== | ==Re-doing analytical digest of pMG.004== | ||
* Only of C1 miniprep from 18/7/12 | * Only of C1 miniprep from 18/7/12 | ||
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Revision as of 09:42, 19 July 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> back to our group notebook <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||
preparing gene transfer assayFollowing Juliens advices and references (....), we founded that b. Subtilis was a good candidate for our gene transfer assay. Even more since we had already strains: there are 3 strains in IGEM2011 glycerol stocks: BSPY79: wild type. SB513: Kan resistant. (stored as S10) SB473: CM resistant. (stroed as S11) Baback is worried that BSPY79 is contaminated. To ckeck it and to obtain a non contaminated new glycerol stock; Analytical digest of pMG.004 miniprep from 18/7/12Expected:
5uL digest with XbaI and PstI 3xMinipreps C1, C2, C3
Re-doing analytical digest of pMG.004
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