IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/19
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preparing gene transfer assayFollowing Juliens advices and references (....), we founded that b. Subtilis was a good candidate for our gene transfer assay. Even more since we had already strains: there are 3 strains in IGEM2011 glycerol stocks: BSPY79: wild type. SB513: Kan resistant. (stored as S10) SB473: CM resistant. (stroed as S11) Baback is worried that BSPY79 is contaminated. Analytical digest of pMG.004 miniprep from 18/7/12To ckeck it and to obtain a non contaminated new glycerol stock; |