IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/26: Difference between revisions

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(Autocreate 2012/07/26 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/modularity_group/day_by_day/)
 
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==Entry title==
==Cell viability plates BamHI pMG.004==
* Insert content here...
*Cultures were induced at different levels


OD of saturated cultures at endpoint, before dilution:
{| class="wikitable"
{| border="1"
|-
! induction arabinose !! OD600
|-
| 1 mM || 2.97
|-
| 100 mM || 3.06
|-
| 0 mM || 3.02
|}
Diluted cultures plated for viability
{| class="wikitable"
{| border="1"
|-
! orig. arabinose induction !! cell dilution
|-
| 1 mM || 1:1
|-
| 1 mM || 1:10
|-
| 1 mM || 1:100
|-
| 100 uM || 1:1
|-
| 100 uM || 1:10
|-
| 100 uM || 1:100
|-
| 0 || 1:1
|-
| 0 || 1:10
|-
| 0 || 1:100
|}
Result: no significant difference between induced and uninduced cultures
==Glycerol stocks of subtilis strains==
*BSPY79: wild type (TET resistant)
*S10 [SB513]: Kan resistant
*S11 [SB473]: Cm resistant
==Luminescence==
*5 mL pMG.002(NEB) grown 2 hours with Cm
*2x100 mL flasks induced with 100 uL arabinose innuculated with 2.5 mL culture
*One flask with Cm, one without
Result: no luminescence visible


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Revision as of 07:41, 11 September 2012

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Cell viability plates BamHI pMG.004

  • Cultures were induced at different levels

OD of saturated cultures at endpoint, before dilution:

induction arabinose OD600
1 mM 2.97
100 mM 3.06
0 mM 3.02


Diluted cultures plated for viability

orig. arabinose induction cell dilution
1 mM 1:1
1 mM 1:10
1 mM 1:100
100 uM 1:1
100 uM 1:10
100 uM 1:100
0 1:1
0 1:10
0 1:100

Result: no significant difference between induced and uninduced cultures

Glycerol stocks of subtilis strains

  • BSPY79: wild type (TET resistant)
  • S10 [SB513]: Kan resistant
  • S11 [SB473]: Cm resistant

Luminescence

  • 5 mL pMG.002(NEB) grown 2 hours with Cm
  • 2x100 mL flasks induced with 100 uL arabinose innuculated with 2.5 mL culture
  • One flask with Cm, one without

Result: no luminescence visible

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