IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/05

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(Autocreate 2012/07/05 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/modularity_group/day_by_day/)
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==Entry title==
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==Transformation Efficiency of NEB Turbo (28/06/2012) Chemically Competent Cells==
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* Insert content here...
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* 10^5 transformants/ug
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* Started 15 mL culture in the morning of NEB Turbo with pSB1C3 (pMG.003)
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==Result of K325909 transformation of 4/7/12==
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* 1 colony on the plate with 200 uL transformed culture added
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* Started 15 mL culture in the morning of NEB Turbo with pSB1C3 (pMG.002)
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==Glycerol stocks and Minipreps of 3 X K381001 (pMG.001) Clones==
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* Stocks made in duplicate (-20C and -80C) of 5 mL liquid cultures of the 3 clones of pMG.001 from 04/07/2012
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* Minipreps of remaining culture
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{| class="wikitable"
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{| border="1"
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|-
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! Culture # !! Conc. DNA
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|-
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| C1 || 119 ng/uL
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|-
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| C2 || 141 ng/uL
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|-
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| C3 || 122 ng/uL
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|}

Revision as of 08:11, 5 July 2012

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Transformation Efficiency of NEB Turbo (28/06/2012) Chemically Competent Cells

  • 10^5 transformants/ug
  • Started 15 mL culture in the morning of NEB Turbo with pSB1C3 (pMG.003)

Result of K325909 transformation of 4/7/12

  • 1 colony on the plate with 200 uL transformed culture added
  • Started 15 mL culture in the morning of NEB Turbo with pSB1C3 (pMG.002)

Glycerol stocks and Minipreps of 3 X K381001 (pMG.001) Clones

  • Stocks made in duplicate (-20C and -80C) of 5 mL liquid cultures of the 3 clones of pMG.001 from 04/07/2012
  • Minipreps of remaining culture
Culture # Conc. DNA
C1 119 ng/uL
C2 141 ng/uL
C3 122 ng/uL


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