IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/06

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(→Biobricks checking)
Current revision (12:53, 6 July 2012) (view source)
(→Biobricks checking)
 
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|-
|-
| PstI|| 0.5 uL
| PstI|| 0.5 uL
 +
|}
 +
 +
{| class="wikitable"
 +
{| border="1"
 +
|-
 +
!  !! Frament 1 (biobrick) !! Frament 2 (pSB1C3)
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|-
 +
| pMG.001 || 997 bp || 2060 bp
 +
|-
 +
| pMG.002 || 7655 bp || 2060 bp
|}
|}

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assay Eglowli

  • no luminescence can be observed in a dark room.

--> we should have induced at 30°C or below. and look at the culture after 5 h of induction.



11h30: beginning of a new culture.


this time:

    • 20ml of fresh LB, 200 μL of the 'old ' culture,
    • 28°C (Tamara s incubator),

and same concentrations of Antibiotic and L arabinose

    • 1,5 μL of L arab 20% (100μM)
    • 20μL of Cm,

Biobricks checking

Analytical digests of pMG.001 (3 x colonies) and pMG.002

Reagent Volume
Plasmid DNA 5 uL
Water 12 uL
FastDigest Buffer 2 uL
XbaI 0.5 uL
PstI 0.5 uL
Frament 1 (biobrick) Frament 2 (pSB1C3)
pMG.001 997 bp 2060 bp
pMG.002 7655 bp 2060 bp


Image:KR003737.BMP‎

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