IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/17

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Current revision (13:03, 17 July 2012) (view source)
 
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* of pMG.004, AKA BBa_I716462
* of pMG.004, AKA BBa_I716462
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* Following jake's protocol
+
* Following jake's protocol ( but heat shock timing not sure... issue with timer)
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Biobrick BBa_I716462

in accordance with discussion with Yifan, we want to use the BamH1Bekerley 2007/ Letherbridge 2011 project. LINK

opening of a new biobrick:

Code Date Received Registry Markers Rep. Origin Backbone Source Description of Function Location Notes
pMG.00417-07-12BBa_I716462ApMB1pSB1A22012 Kit Plate 2 3CBamHI endonuclease under pBAD

( it is registered in the "online following sheet")

Transformation

  • of pMG.004, AKA BBa_I716462
  • Following jake's protocol ( but heat shock timing not sure... issue with timer)
Plate Plasmid Dilution
1 BBa_I716462 from registry 1:1
2 BBa_I716462 from registry 1:10
3 lacZ on pSB1A2 108 ng/uL 1:1
4 lacZ on pSB1A2 108 ng/uL 1:10
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