IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/18

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Presence of .... colonies in the 1/1 petri dish. Nothing in the 1/10.  
Presence of .... colonies in the 1/1 petri dish. Nothing in the 1/10.  
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==minipreping and glycerol stocks==
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==miniprepping and glycerol stocks of pMG.004==
'''12h: launching of 12mL cultures in 50 mL flasks''':  
'''12h: launching of 12mL cultures in 50 mL flasks''':  
3 cultures from 3 distinct colonies of pmg.004/NEB turbo culture. (LB + 12μL Ampicilline)
3 cultures from 3 distinct colonies of pmg.004/NEB turbo culture. (LB + 12μL Ampicilline)
 +
 +
Miniprep plasmid stock
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 +
{| class="wikitable"
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{| border="1"
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|-
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! Clone !! Concentration [ng/uL]
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|-
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| C1 || 92.7
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|-
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| C2 || 121.8
 +
|-
 +
| C3 || 126.8
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|}
 +
 +
==Glycerol stocks of TOP10==
 +
3 cultures of top 10.( LB + 6μL Spectomycine)
3 cultures of top 10.( LB + 6μL Spectomycine)
 +
3 glycerol stocks of each (1 each for Chantal, 2 for us)
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Slow growing culture, too much antibiotic?
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==Launched culture for TOP10 competent cells==
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*20mL with Streptomycin
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 +
==discution with Julien: 2nd round==
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}
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==discution with Julien: 2nd round==
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__NOTOC__
__NOTOC__
[[category:OWWLabNotebookV1]]
[[category:OWWLabNotebookV1]]

Revision as of 09:37, 19 July 2012

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transformation results

control OK. Presence of .... colonies in the 1/1 petri dish. Nothing in the 1/10.

miniprepping and glycerol stocks of pMG.004

12h: launching of 12mL cultures in 50 mL flasks: 3 cultures from 3 distinct colonies of pmg.004/NEB turbo culture. (LB + 12μL Ampicilline)

Miniprep plasmid stock

Clone Concentration [ng/uL]
C1 92.7
C2 121.8
C3 126.8

Glycerol stocks of TOP10

3 cultures of top 10.( LB + 6μL Spectomycine) 3 glycerol stocks of each (1 each for Chantal, 2 for us) Slow growing culture, too much antibiotic?

Launched culture for TOP10 competent cells

  • 20mL with Streptomycin

discution with Julien: 2nd round

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