IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/18

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Current revision (09:22, 19 July 2012) (view source)
 
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Presence of .... colonies in the 1/1 petri dish. Nothing in the 1/10.  
Presence of .... colonies in the 1/1 petri dish. Nothing in the 1/10.  
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==miniprepping and glycerol stocks of pMG.004==
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==miniprepping and glycerol stocks==
'''12h: launching of 12mL cultures in 50 mL flasks''':  
'''12h: launching of 12mL cultures in 50 mL flasks''':  
3 cultures from 3 distinct colonies of pmg.004/NEB turbo culture. (LB + 12μL Ampicilline)
3 cultures from 3 distinct colonies of pmg.004/NEB turbo culture. (LB + 12μL Ampicilline)
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3 cultures of top 10.( LB + 6μL Spectomycine)
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Miniprep plasmid stock
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==discussion with Julien: 2nd round==
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{| class="wikitable"
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==Researching natural competence/cross species expression==
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{| border="1"
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[http://opus.bibliothek.uni-wuerzburg.de/volltexte/2011/6060/pdf/Kreft13.pdf]
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! Clone !! Concentration [ng/uL]
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| C1 || 92.7
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| C2 || 121.8
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| C3 || 126.8
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|}
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==Glycerol stocks of TOP10==
 
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3 cultures of top 10.( LB + 6μL Spectomycine)
 
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3 glycerol stocks of each (1 each for Chantal, 2 for us)
 
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Slow growing culture, too much antibiotic?
 
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==Launched culture for TOP10 competent cells==
 
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*20mL with Streptomycin
 
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==discution with Julien: 2nd round==
 
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Current revision

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transformation results

control OK. Presence of .... colonies in the 1/1 petri dish. Nothing in the 1/10.

miniprepping and glycerol stocks

12h: launching of 12mL cultures in 50 mL flasks: 3 cultures from 3 distinct colonies of pmg.004/NEB turbo culture. (LB + 12μL Ampicilline) 3 cultures of top 10.( LB + 6μL Spectomycine)

discussion with Julien: 2nd round

Researching natural competence/cross species expression

[1]


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