IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/19

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Baback is worried that BSPY79 is contaminated.  
Baback is worried that BSPY79 is contaminated.  
 +
To ckeck it and to obtain a non contaminated new glycerol stock;
==Analytical digest of pMG.004 miniprep from 18/7/12==
==Analytical digest of pMG.004 miniprep from 18/7/12==
 +
Expected:
 +
* 2020 bp backbone pST1A2
 +
* 1940 bp insert BBa_I716462
 +
5uL digest with XbaI and PstI 3xMinipreps C1, C2, C3
 +
[[image:KR003829.png]]
 +
*Bad result, what is the problem? It's as though it is 1/2 digested but the size is too small (would be 4kb)
 +
 +
==Re-doing analytical digest of pMG.004==
 +
* Only of C1 miniprep from 18/7/12
 +
*
 +
 +
{| class="wikitable"
 +
{| border="1"
 +
|-
 +
!  !! XbaI !! PstI
 +
|-
 +
| 1 || X ||
 +
|-
 +
| 2 ||  || X
 +
|-
 +
| 3 || X || X
 +
|-
 +
| 4 ||  ||
 +
|}
-
To ckeck it and to obtain a non contaminated new glycerol stock;
 
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Revision as of 11:41, 19 July 2012

Project name back to our group notebook
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preparing gene transfer assay

Following Juliens advices and references (....), we founded that b. Subtilis was a good candidate for our gene transfer assay. Even more since we had already strains: there are 3 strains in IGEM2011 glycerol stocks:

BSPY79: wild type. SB513: Kan resistant. (stored as S10) SB473: CM resistant. (stroed as S11)

Baback is worried that BSPY79 is contaminated. To ckeck it and to obtain a non contaminated new glycerol stock;

Analytical digest of pMG.004 miniprep from 18/7/12

Expected:

  • 2020 bp backbone pST1A2
  • 1940 bp insert BBa_I716462

5uL digest with XbaI and PstI 3xMinipreps C1, C2, C3 image:KR003829.png

  • Bad result, what is the problem? It's as though it is 1/2 digested but the size is too small (would be 4kb)

Re-doing analytical digest of pMG.004

  • Only of C1 miniprep from 18/7/12
XbaI PstI
1 X
2 X
3 X X
4


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