IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/20

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(Autocreate 2012/07/20 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/modularity_group/day_by_day/)
(Entry title)
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==Entry title==
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==Transformation with new TOP10 CaCl2 competent cells from 19/07/12==
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* Insert content here...
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* 1 uL of DNA added to each
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{| class="wikitable"
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{| border="1"
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|-
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! Plate !! Plasmid !! Concentration DNA [ng/uL] !! Volume plated [uL]
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|-
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| 1 || pSB1C3 || 100 || 200
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|-
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| 2 || pSB1C3 || 10 || 200
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|-
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| 3 || pSB1C3 || 100 || 200
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|-
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| 4 || pSB1C3 || 10 || 200
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|-
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| 5 || pMG.004 || 202 || 200
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|-
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| 6 || pMG.004 || 202 || 20
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|}
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==Analytical digest of pMG.004 round 3==
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*To discover is there is bad XbaI or bad plasmid
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{| class="wikitable"
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{| border="1"
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|-
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! Plate !! Plasmid
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|-
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| XbaI tube 1 + PstI || pMG.004 2uL
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|-
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| XbaI tube 2 + PstI || pMG.004 2uL
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|-
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| XbaI tube 3 + PstI || pMG.004 2uL
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|-
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| XbaI tube 4 + PstI || pMG.004 2uL
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|-
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| NotI || pMG.004 2uL
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|}
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Revision as of 11:59, 20 July 2012

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Transformation with new TOP10 CaCl2 competent cells from 19/07/12

  • 1 uL of DNA added to each
Plate Plasmid Concentration DNA [ng/uL] Volume plated [uL]
1 pSB1C3 100 200
2 pSB1C3 10 200
3 pSB1C3 100 200
4 pSB1C3 10 200
5 pMG.004 202 200
6 pMG.004 202 20

Analytical digest of pMG.004 round 3

  • To discover is there is bad XbaI or bad plasmid
Plate Plasmid
XbaI tube 1 + PstI pMG.004 2uL
XbaI tube 2 + PstI pMG.004 2uL
XbaI tube 3 + PstI pMG.004 2uL
XbaI tube 4 + PstI pMG.004 2uL
NotI pMG.004 2uL
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