IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/20
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(Autocreate 2012/07/20 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/modularity_group/day_by_day/) |
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| - | == | + | ==Transformation with new TOP10 CaCl2 competent cells from 19/07/12== |
| - | + | ||
| + | * 1 uL of DNA added to each | ||
| + | |||
| + | {| class="wikitable" | ||
| + | {| border="1" | ||
| + | |- | ||
| + | ! Plate !! Plasmid !! Concentration DNA [ng/uL] !! Volume plated [uL] | ||
| + | |- | ||
| + | | 1 || pSB1C3 || 100 || 200 | ||
| + | |- | ||
| + | | 2 || pSB1C3 || 10 || 200 | ||
| + | |- | ||
| + | | 3 || pSB1C3 || 100 || 200 | ||
| + | |- | ||
| + | | 4 || pSB1C3 || 10 || 200 | ||
| + | |- | ||
| + | | 5 || pMG.004 || 202 || 200 | ||
| + | |- | ||
| + | | 6 || pMG.004 || 202 || 20 | ||
| + | |} | ||
| + | |||
| + | ==Analytical digest of pMG.004 round 3== | ||
| + | *To discover is there is bad XbaI or bad plasmid | ||
| + | |||
| + | {| class="wikitable" | ||
| + | {| border="1" | ||
| + | |- | ||
| + | ! Plate !! Plasmid | ||
| + | |- | ||
| + | | XbaI tube 1 + PstI || pMG.004 2uL | ||
| + | |- | ||
| + | | XbaI tube 2 + PstI || pMG.004 2uL | ||
| + | |- | ||
| + | | XbaI tube 3 + PstI || pMG.004 2uL | ||
| + | |- | ||
| + | | XbaI tube 4 + PstI || pMG.004 2uL | ||
| + | |- | ||
| + | | NotI || pMG.004 2uL | ||
| + | |} | ||
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Revision as of 12:59, 20 July 2012
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Transformation with new TOP10 CaCl2 competent cells from 19/07/12
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