IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/25

Current revision

Project name

20 mL LB (no antibiotic) inoculated with 500 uL log phase cells grown in Cm
Induced upon inoculation with arabinose
- 1.5 uL arabinose in 20 mL : 100uM
- 15 uL arabinose in 20 mL : 1 mM
Grown overnight with shaking at 30C
Exposed to oxygen in the morning and shaken vigorously
Lightly visible luminescence when in darkness

intended arabinose induction	LB Volume [mL]	Added arab.
1.0 mM	3	2.5 uL
100 uM	3	0.3 uL
0	3	0

S10 [SB513] (Kan resistant) and S11 [SB473] (Cm resistant) taken from Paris 2011 freezer stocks
BSPY79 (wild type, Tet resistant) taken from Sarahs Tet plates
Grown in LB overnight

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-==Entry title==
+==Luminescence test of pMG.002 in TOP10==
-* Insert content here...
+* 20 mL LB (no antibiotic) inoculated with 500 uL log phase cells grown in Cm
+* Induced upon inoculation with arabinose
+** 1.5 uL arabinose in 20 mL : 100uM
+** 15 uL arabinose in 20 mL : 1 mM
+* Grown overnight with shaking at 30C
+* Exposed to oxygen in the morning and shaken vigorously
+* Lightly visible luminescence when in darkness
+==BamHI cell viability test==
+* Grow to OD of 0.6
+* Induce with arabinose
+* Grow overnight
+* Dilute and plate cells
+{| class="wikitable"
+{| border="1"
+|-
+! intended arabinose induction !! LB Volume [mL] !! Added arab.
+|-
+| 1.0 mM || 3 || 2.5 uL
+|-
+| 100 uM || 3 ||0.3 uL
+|-
+| 0 || 3 || 0
+|}
+==Liquid cultures of subtilis strains for glycerol==
+* S10 [SB513] (Kan resistant) and S11 [SB473] (Cm resistant) taken from Paris 2011 freezer stocks
+* BSPY79 (wild type, Tet resistant) taken from Sarahs Tet plates
+* Grown in LB overnight
 <!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
 |}