IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/25

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(Autocreate 2012/07/25 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/modularity_group/day_by_day/)
Current revision (11:28, 26 July 2012) (view source)
(Entry title)
 
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==Entry title==
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==Luminescence test of pMG.002 in TOP10==
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* Insert content here...
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* 20 mL LB (no antibiotic) inoculated with 500 uL log phase cells grown in Cm
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* Induced upon inoculation with arabinose
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** 1.5 uL arabinose in 20 mL : 100uM
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** 15 uL arabinose in 20 mL : 1 mM
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* Grown overnight with shaking at 30C
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* Exposed to oxygen in the morning and shaken vigorously
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* Lightly visible luminescence when in darkness
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==BamHI cell viability test==
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* Grow to OD of 0.6
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* Induce with arabinose
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* Grow overnight
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* Dilute and plate cells
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{| class="wikitable"
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{| border="1"
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|-
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! intended arabinose induction !! LB Volume [mL] !! Added arab.
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|-
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| 1.0 mM || 3 || 2.5 uL
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|-
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| 100 uM || 3 ||0.3 uL
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|-
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| 0 || 3 || 0
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|}
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==Liquid cultures of subtilis strains for glycerol==
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* S10 [SB513] (Kan resistant) and S11 [SB473] (Cm resistant) taken from Paris 2011 freezer stocks
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* BSPY79 (wild type, Tet resistant) taken from Sarahs Tet plates
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* Grown in LB overnight
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Luminescence test of pMG.002 in TOP10

  • 20 mL LB (no antibiotic) inoculated with 500 uL log phase cells grown in Cm
  • Induced upon inoculation with arabinose
    • 1.5 uL arabinose in 20 mL : 100uM
    • 15 uL arabinose in 20 mL : 1 mM
  • Grown overnight with shaking at 30C
  • Exposed to oxygen in the morning and shaken vigorously
  • Lightly visible luminescence when in darkness

BamHI cell viability test

  • Grow to OD of 0.6
  • Induce with arabinose
  • Grow overnight
  • Dilute and plate cells
intended arabinose induction LB Volume [mL] Added arab.
1.0 mM 3 2.5 uL
100 uM 3 0.3 uL
0 3 0

Liquid cultures of subtilis strains for glycerol

  • S10 [SB513] (Kan resistant) and S11 [SB473] (Cm resistant) taken from Paris 2011 freezer stocks
  • BSPY79 (wild type, Tet resistant) taken from Sarahs Tet plates
  • Grown in LB overnight
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