IGEM:Paris Bettencourt 2012/Notebooks/modularity group/day by day//2012/07/26

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Current revision (09:45, 11 September 2012) (view source)
 
Line 56: Line 56:
==Luminescence==
==Luminescence==
-
*5 mL pMG.002(NEB) grown 2 hours with Cm
+
*5 mL pMG.002(Top10) grown 2 hours with Cm
*2x100 mL flasks induced with 100 uL arabinose innuculated with 2.5 mL culture
*2x100 mL flasks induced with 100 uL arabinose innuculated with 2.5 mL culture
*One flask with Cm, one without
*One flask with Cm, one without
-
Result: no luminescence visible
+
Result: both luminesce
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Cell viability plates BamHI pMG.004

  • Cultures were induced at different levels

OD of saturated cultures at endpoint, before dilution:

induction arabinose OD600
1 mM 2.97
100 mM 3.06
0 mM 3.02


Diluted cultures plated for viability

orig. arabinose induction cell dilution
1 mM 1:1
1 mM 1:10
1 mM 1:100
100 uM 1:1
100 uM 1:10
100 uM 1:100
0 1:1
0 1:10
0 1:100

Result: no significant difference between induced and uninduced cultures

Glycerol stocks of subtilis strains

  • BSPY79: wild type (TET resistant)
  • S10 [SB513]: Kan resistant
  • S11 [SB473]: Cm resistant

Luminescence

  • 5 mL pMG.002(Top10) grown 2 hours with Cm
  • 2x100 mL flasks induced with 100 uL arabinose innuculated with 2.5 mL culture
  • One flask with Cm, one without

Result: both luminesce

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