IGEM:Paris Bettencourt 2012/Notebooks/suicide group
From OpenWetWare
The aim of this subproject is to design an effective killing mechanism for the bacteria. We design a self killing mechanism using Colicin toxin-antitoxin system.
Colicins
Image taken from: Cascales E, et al. (2007) Colicin biology. Microbiol Mol Biol Rev71:158–229.
Parts
- BBa_K131000 Colicin E2 operon, designed by Kevin McLeod, group: iGEM08_Calgary_Wetware (2008-07-22)
- BBa_K117001 Colicin E7 with immunity, designed by Nguyen Xuan Hung, group: iGEM08_NTU-Singapore (2008-10-07)
- BBa_K117000 Lysis gene (promotes lysis in colicin-producing bacteria strain), designed by Nguyen Xuan Hung, group: iGEM08_NTU-Singapore (2008-10-07)
Promoters
- BBa_R0040 TetR repressible promoter, designed by Designed by June Rhee, Connie Tao, Ty Thomson, Louis Waldman, group: Registry (2003-01-31)
- BBa_R0011 Promoter (lacI regulated, lambda pL hybrid), designed by Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton, group: Registry (2003-01-31)
References
- Cascales E, et al. (2007) Colicin biology. Microbiol Mol Biol Rev71:158–229.