IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/07/11: Difference between revisions

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==What we did today==
==What we did today==
*Ligation (after digested all with X and P):
*Ligation (after digested all with X and P):
**pSG001 as negative control
**pSG.001 as negative control
**pSG002 as negative control
**pSG.002 as negative control
**pSG005 (pSG001+pRG006 (pLac from Antoine))
**pSG.005 (pSG.001+pRG.006 (pLac from Antoine))
**psG006 (pSG002+pSG004 (pTet from kit))
**psG.006 (pSG.002+pSG.004 (pTet from kit))
*Transforming the ligation product and plating
*Transforming the ligation product and plating (plus 1 positive control)
*Digesting pSG004 and pRG006 with X and P
*Digesting pSG.004 and pRG.006 with X and P
[[Image:110712.jpg]]
[[Image:110712.jpg]]
*Growing more pSG004 and pRG006
*Growing more pSG.004 and pRG.006




Revision as of 02:07, 12 July 2012

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What we did today

  • Ligation (after digested all with X and P):
    • pSG.001 as negative control
    • pSG.002 as negative control
    • pSG.005 (pSG.001+pRG.006 (pLac from Antoine))
    • psG.006 (pSG.002+pSG.004 (pTet from kit))
  • Transforming the ligation product and plating (plus 1 positive control)
  • Digesting pSG.004 and pRG.006 with X and P

  • Growing more pSG.004 and pRG.006


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