IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/07/19: Difference between revisions

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PCR protocol
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! Step !! Temp !! Time
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| 1 || 95 || 1 min
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| 2 || 95 || 30 sec
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| 3 || 55 || 30 sec
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| 4 || 72 || 2 min
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| 5 || Goto 2 rep || 30x
|-
| 6 || 72 || 5 min
|-
| 7 || 10 || HOLD
|}
PCR results
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Revision as of 08:49, 19 July 2012

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PCR of recovered BBa_K131009 (pSG.012)

Reagent Volume
Template DNA 0.2 uL (~70ng)
Water 23.8 uL
PCR MM 25 uL
10uM primer 1 uL


PCR protocol

Step Temp Time
1 95 1 min
2 95 30 sec
3 55 30 sec
4 72 2 min
5 Goto 2 rep 30x
6 72 5 min
7 10 HOLD


PCR results

Name Fragments (PCR product) Primers
pSG.007 1.8 kb iSG.003+iSG.004
pSG.008 300 bp iSG.005+iSG.006

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