IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/07/19: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 54: Line 54:
! Name !! Fragments (PCR product) !! Primers
! Name !! Fragments (PCR product) !! Primers
|-
|-
| pSG.013 || 1.8 kb || iSG.003+iSG.004
| pSG.013 || 1746 bp || iSG.003+iSG.004
|-
|-
| pSG.014 || 300 bp || iSG.005+iSG.006
| pSG.014 || 261 bp || iSG.005+iSG.006
|}
|}


Revision as of 06:21, 20 July 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

  • Insert content here...

PCR of recovered BBa_K131009 (pSG.012)

Reagent Volume
Template DNA 0.2 uL (~70ng)
Water 23.8 uL
PCR MM 25 uL
10uM primer 1 uL


PCR protocol

Step Temp Time
1 95 1 min
2 95 30 sec
3 55 30 sec
4 72 2 min
5 Goto 2 rep 30x
6 72 5 min
7 10 HOLD


PCR results

Name Fragments (PCR product) Primers
pSG.013 1746 bp iSG.003+iSG.004
pSG.014 261 bp iSG.005+iSG.006

Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Paris_Bettencourt_2012/Notebooks/suicide_group/day_by_day//2012/07/19&oldid=614883"