IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/07/19

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PCR Colicin Activity and Immunity

PCR of recovered BBa_K131009 (pSG.012)

Reagent Volume
Template DNA 0.2 uL (~70ng)
Water 23.8 uL
PCR MM 25 uL
10uM primer 1 uL


PCR protocol

Step Temp Time
1 95 1 min
2 95 30 sec
3 55 30 sec
4 72 2 min
5 Goto 2 rep 30x
6 72 5 min
7 10 HOLD


PCR results

Name Fragments (PCR product) Primers
pSG.013 1746 bp iSG.003+iSG.004
pSG.014 261 bp iSG.005+iSG.006

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