IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/07/20: Difference between revisions
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== | ==What we did today== | ||
We want to insert Colicin immunity and activity inside the plasmid with promoter that we want, so we digested pSG.005 (pLac inside pSB3C5) with SpeI and PstI and pSG.013, pSG.014 (toxin, immunity) both with XbaI and PstI. | |||
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Revision as of 15:30, 24 September 2012
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||
What we did todayWe want to insert Colicin immunity and activity inside the plasmid with promoter that we want, so we digested pSG.005 (pLac inside pSB3C5) with SpeI and PstI and pSG.013, pSG.014 (toxin, immunity) both with XbaI and PstI.
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