IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/10: Difference between revisions

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(Autocreate 2012/08/10 Entry for IGEM:Paris_Bettencourt_2012/Notebooks/suicide_group/day_by_day/)
 
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==Entry title==
==Digestion of pSG.021(?)==
* Insert content here...
* We did digestion of pSG.021 (pLac - ColE2Imm) plasmids from the 2nd colony with X and P.
* The expected size of insert is about 300bp.
 
===Protocol===
 
{| style="border-spacing:0;"
| style="border-top:0.002cm solid #000000;border-bottom:0.002cm solid #000000;border-left:0.002cm solid #000000;border-right:none;padding:0.097cm;"| Plasmid
| style="border:0.002cm solid #000000;padding:0.097cm;"| 6 uL
 
|-
| style="border-top:none;border-bottom:0.002cm solid #000000;border-left:0.002cm solid #000000;border-right:none;padding:0.097cm;"| H2O
| style="border-top:none;border-bottom:0.002cm solid #000000;border-left:0.002cm solid #000000;border-right:0.002cm solid #000000;padding:0.097cm;"| 26 uL
 
|-
| style="border-top:none;border-bottom:0.002cm solid #000000;border-left:0.002cm solid #000000;border-right:none;padding:0.097cm;"| FD 10x Green Buffer
| style="border-top:none;border-bottom:0.002cm solid #000000;border-left:0.002cm solid #000000;border-right:0.002cm solid #000000;padding:0.097cm;"| 4 uL
 
|-
| style="border-top:none;border-bottom:0.002cm solid #000000;border-left:0.002cm solid #000000;border-right:none;padding:0.097cm;"| XbaI
| style="border-top:none;border-bottom:0.002cm solid #000000;border-left:0.002cm solid #000000;border-right:0.002cm solid #000000;padding:0.097cm;"| 2 uL
 
|-
| style="border-top:none;border-bottom:0.002cm solid #000000;border-left:0.002cm solid #000000;border-right:none;padding:0.097cm;"| PstI
| style="border-top:none;border-bottom:0.002cm solid #000000;border-left:0.002cm solid #000000;border-right:0.002cm solid #000000;padding:0.097cm;"| 2 uL
 
|-
| style="border-top:none;border-bottom:0.002cm solid #000000;border-left:0.002cm solid #000000;border-right:none;padding:0.097cm;"| Total
| style="border-top:none;border-bottom:0.002cm solid #000000;border-left:0.002cm solid #000000;border-right:0.002cm solid #000000;padding:0.097cm;"| 40 uL
 
|}
 
Incubate 1h15 with shaking at 37°C.
 
===Result===
 
[[Image:100812.png]]




Revision as of 08:55, 13 August 2012

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Digestion of pSG.021(?)

  • We did digestion of pSG.021 (pLac - ColE2Imm) plasmids from the 2nd colony with X and P.
  • The expected size of insert is about 300bp.

Protocol

Plasmid 6 uL
H2O 26 uL
FD 10x Green Buffer 4 uL
XbaI 2 uL
PstI 2 uL
Total 40 uL

Incubate 1h15 with shaking at 37°C.

Result


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