IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/10: Difference between revisions

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So ok, it is NOT the immunity gene.
So ok, it is NOT the immunity gene.

Revision as of 15:36, 24 September 2012

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Digestion of pSG.021(?)

  • We did digestion of pSG.021 (pLac - ColE2Imm) plasmids from the 2nd colony with X and P.
  • The expected size of insert is about 300bp.

Protocol

Plasmid 6 uL
H2O 26 uL
FD 10x Green Buffer 4 uL
XbaI 2 uL
PstI 2 uL
Total 40 uL

Incubate 1h15 with shaking at 37°C.

Result

... So ok, it is NOT the immunity gene.

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