IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/16: Difference between revisions
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==Colony PCR== | ==Colony PCR== |
Revision as of 09:43, 17 August 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Colony PCRWe did colony PCR for all the colonies of pSG.008 and pSG.021 Colony+50μL H2O:
Protocol
PCR Program
Ligation of pTetWe ligated pTet (annealing product) with pSG.002 (E,S), Kan resistant Protocol
Incubate in 20°C (microscope room) for 1h |