IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/21

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Restriction Digest

  • Insert content here...
Reagent pSG.002
DNA 7 uL
Buffer 10x 4 uL
XbaI 2 uL
PstI 2 uL
H2O 25 uL
Total 40 uL



Ligation

Reagent pSG.024 pSG.002 -
Vector pSG.002 2 uL pSG.002 2 uL
Insert pSG.013 6.5 uL -
Buffer 10X 1 uL 1 uL
T4 DNA ligase 0.5 uL 0.5 uL
H2O 0 uL 6.5
Total 10 uL 10 uL
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