IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/22
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Colicin Assay
|
Ligation
Reagent | pSG.024A | pSG.024B | PSG.002B - |
Vector | pSG.002A 1 uL | pSG.002B 1.5 uL | pSG.002B 1 uL |
Insert | pSG.013 3 uL | pSG.013 uL | - |
Buffer 10x | 1 uL | 1 uL | 1 uL |
T4 DNA Ligase | 0.5 uL | 0.5 uL | 0.5 uL |
H2O | 4.5 uL | 4 uL | 4.5 uL |
Total | 10 uL | 10 uL | 10 uL |