IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/09/21
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pLac + Colicin E2 Immunity CharacterizationWe want to characterize the immunity by varying the concentration of IPTG and the ColicinE2 cells. Protocol
Note: 1ml of cells of OD 1.0 has 1e9 cells, so we expect if all cells survive we will get 10000 colonies at the end. ResultsThe table shows the number of colonies survive in each plate IPTG x Colicin. This time we had too many colonies (very difficult to count), and still did not show the effect of Colicin and IPTG. The next experiment we would add another control which is cell with Cm resistance (pSB3C5) but no immunity gene, and do more dilution (and more carefully).
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