IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/07/20

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Current revision (18:30, 24 September 2012) (view source)
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==Entry title==
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==What we did today==
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We want to insert Colicin immunity and activity inside the plasmid with promoter that we want, so we digested pSG.005 (pLac inside pSB3C5) with SpeI and PstI and pSG.013, pSG.014 (toxin, immunity) both with XbaI and PstI.
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What we did today

We want to insert Colicin immunity and activity inside the plasmid with promoter that we want, so we digested pSG.005 (pLac inside pSB3C5) with SpeI and PstI and pSG.013, pSG.014 (toxin, immunity) both with XbaI and PstI.

Reagent pSG.005 pSG.013 pSG.014
Green Buffer 10X 4 uL 4 uL 4 uL
XbaI - 1 uL 1 uL
PstI 1 uL 1 uL 1 uL
SpeI 1 uL - -
FastAP 1 uL - -
DNA 7 uL (~1 ug) 25 uL (~1 ug) 25 uL (~1 ug)
H2O 26 uL 9 uL 9 uL


Name Fragments (Digest product) Restriction Enzyme
pSG.005 3.4 kb SpeI, PstI
pSG.013 1746 bp XbaI, PstI
pSG.014 261 bp XbaI, PstI

Image:SGgel200712-2.jpg Image:SGgel200712-1.jpg

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