IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/07/23

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(Entry title)
Current revision (17:32, 24 September 2012) (view source)
(Entry title)
 
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==Entry title==
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==Ligation==
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We want to put pTet (pSG.004) inside the plasmid pSB4k5 (pSG.002) to make pSG.006.
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pSG.006 Ligation
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{| style="border-spacing:0;"
{| style="border-spacing:0;"
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| Reagent
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| Reagent

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Ligation

We want to put pTet (pSG.004) inside the plasmid pSB4k5 (pSG.002) to make pSG.006.

Reagent Volume
pSG.004 X,P 0.5 uL
pSG.002 X,P 2 uL
Buffer 10x 1 uL
T4 DNA Ligase 0.5 uL
H2O 6 uL

Transformation

Recovery time: 2 hrs Plates: 1 normal Kan, 1 LB + 10 uL Kan gradient


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