IGEM:Paris Bettencourt 2012/Notebooks/suicide group/day by day//2012/08/21

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(Entry title)
Current revision (07:37, 17 September 2012) (view source)
(Restriction Digest)
 
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==Restriction Digest==
==Restriction Digest==
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* Insert content here...
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{| style="border-spacing:0;"
{| style="border-spacing:0;"
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| Reagent
| style="border-top:0.0007in solid #000000;border-bottom:0.0007in solid #000000;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| Reagent

Current revision

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Restriction Digest

Reagent pSG.002
DNA 7 uL
Buffer 10x 4 uL
XbaI 2 uL
PstI 2 uL
H2O 25 uL
Total 40 uL



Image:SGgel210812.jpg

Ligation

Reagent pSG.024 pSG.002 -
Vector pSG.002 2 uL pSG.002 2 uL
Insert pSG.013 6.5 uL -
Buffer 10X 1 uL 1 uL
T4 DNA ligase 0.5 uL 0.5 uL
H2O 0 uL 6.5
Total 10 uL 10 uL
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